Čes. stomatol. Prakt. zub. lék. (Czech Dental Journal) 2017; 117(3): 60-67 | DOI: 10.51479/cspzl.2017.010

The Study of Salivary Proteins Using Proteomic Methods in Periodontal Diseases

V. Schwartzová1,*, G. Laputková2, J. Sabo2
1 I. stomatologická klinika, Univerzita P. J. Šafárika, LF, Košice, Slovensko
2 Ústav lekárskej a klinickej biofyziky, Univerzita P. J. Šafárika, LF, Košice, Slovensko

Background: The aim of the present work is to give a brief overview of the latest achievements in the study of salivary proteins by proteomic methods in periodontal diseases.
Traditional diagnostic methods based on visual assessment and radiographic infomations about periodontal status fail to identify people at risk of developing the disease and in determining the degree of susceptibility to disease progression.
Recently, there was when examining body fluids, including saliva analysis, a significant growth in the use of proteomic methods to become an additional tool in the diagnosis of periodontal disease. In the search for potential biomarkers saliva provides a number of advantages including the availability of samples, the possibility of non-invasive sampling and complexity of protein content.
It is therefore a challenge to establish criteria for the diagnosis of periodontal diseases, which are based on evidence (evidence-based criteria). This approach, which uses technology based on mass spectrometry is able to identify and quantify large numbers of proteins and their isoforms in one comprehensive oral fluid samples and extract detailed information on the regulatory mechanisms of the host at the protein level.

Conclusion: The clinical diagnosis based on the analysis of oral fluids, especially one that uses proteomic method allows to determine the relationship between salivary proteins and the condition. Therefore, the purpose of this article attempt to provide an update on the study of salivary proteins proteomic methods and their suitability for use as biomarkers for diagnosing periodontal diseases.

Keywords: saliva; proteomic methods; periodontal diseases

Published: September 1, 2017  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Schwartzová V, Laputková G, Sabo J. The Study of Salivary Proteins Using Proteomic Methods in Periodontal Diseases. Čes. stomatol. Prakt. zub. lék. 2017;117(3):60-67. doi: 10.51479/cspzl.2017.010.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. AlJehani, Y. A.: Risk factors of periodontal disease: review of the literature. Int. J. Dent., 2014, s. 1-9. Go to original source... Go to PubMed...
    2. Anceriz, N., Vandal, K., Tessier, P. A.: S100A9 mediates neutrophil adhesion to fibronectin through activation of β2 integrins. Biochem. Biophys. Res. Commun., roč. 354, 2007, č. 1, s. 84-89. Go to original source... Go to PubMed...
    3. Andersen, E., Dessaix, I. M., Perneger, T., Mombelli, A.: Myeloid-related protein (MRP8/14) expression in gingival crevice fluid in periodontal health and disease and after treatment. J. Periodontal Res., roč. 45, 2010, č. 4, s. 458-463. Go to original source... Go to PubMed...
    4. Giannobile, W. V., Al-Shammari, K. F., Sarment, D. P.: Matrix molecules and growth factors as indicators of periodontal disease activity. Periodontology, 2000, roč. 31, 2003, č. 1, s. 125-134. Go to original source... Go to PubMed...
    5. Giannobile, W. V., Beikler, T., Kinney, J. S., Ramseier, C. A., Morelli, T., Wong, D. T.: Saliva as a diagnostic tool for periodontal disease: current state and future directions. Periodontology, 2000, roč. 50, 2009, č. 1, s. 52-64. Go to original source... Go to PubMed...
    6. Goncalves, LdaR., Soares, M. R., Nogueira, F. C. et al.: Comparative proteomic analysis of whole saliva from chronic periodontitis patients. J. Proteomics, roč. 73, 2010, č. 7, s. 1334-1341. Go to original source... Go to PubMed...
    7. Golub, L. M., Lee, H. M., Greenwald, R. A., Ryan, M. E., Sorsa, T., Salo, T., Giannobile, W. V.: A matrix metalloproteinase inhibitor reduces bone-type collagen degradation fragments and specific collagenases in gingival crevicular fluid during adult periodontitis. Inflamm. Res., roč. 46, 1997, č. 8, s. 310-319. Go to original source... Go to PubMed...
    8. Gursoy, U. K., Könönen, E., Huumonen, S., Tervahartiala, T., Pussinen, P. J., Suominen, A. L., Sorsa, T.: Salivary type I collagen degradation end-products and related matrix metalloproteinases in periodontitis. J. Clin. Periodontol., roč. 40, 2013, č. 1, s. 18-25. Go to original source... Go to PubMed...
    9. Haigh, B. J., Stewart, K. W., Whelan, J. R., Barnett, M. P., Smolenski, G. A., Wheeler, T. T.: Alterations in the salivary proteome associated with periodontitis. J. Clin. Periodontol., roč. 37, 2010, č. 3, s. 241-247. Go to original source... Go to PubMed...
    10. Henskens, Y. M., Veerman, E. C., Nieuw Amerongen, A. V.: Cystatins in health and disease. Biol. Chem. Hoppe Seyler, roč. 377, 1996, č. 2, s. 71-86.
    11. Iwata, T., Kantarci, A., Yagi, M., Jackson, T., Hasturk, H., Kurihara, H., Van Dyke, T. E.: Ceruloplasmin induces polymorphonuclear leukocyte priming in localized aggressive periodontitis. J. Periodontol., roč. 80, 2009, č. 8, s. 1300-1306. Go to original source... Go to PubMed...
    12. Javed, F., Ahmed, H. B., Saeed, A., Mehmood, A., Bain, C.: Whole salivary interleukin-6 and matrix metalloproteinase-8 levels in patients with chronic periodontitis with and without prediabetes. J. Periodontol., roč. 85, 2014, č. 5, s. 130-135. Go to original source... Go to PubMed...
    13. Khiste, S. V., Ranganath, V., Nichani, A. S., Rajani, V.: Critical analysis of biomarkers in the current periodontal practice. J. Indian Soc. Periodontol., roč. 15, 2011, č. 2, s. 104. Go to original source... Go to PubMed...
    14. Kojima, T., Andersen, E., Sanchez, J. C., Wilkins, M. R., Hochstrasser, D. F., Pralong, W. F., Cimasoni, G.: Human gingival crevicular fluid contains MRP8 (S100A8) and MRP14 (S100A9), two calcium-binding proteins of the S100 family. J. Dent. Res., roč. 79, 2000, č. 2, s. 740-747. Go to original source... Go to PubMed...
    15. Listgarten, M. A., Loomer, P. M.: Microbial identification in the management of periodontal diseases. A systematic review. Ann. Periodontol., roč. 8, 2003, č. 1, s. 182-192. Go to original source... Go to PubMed...
    16. Mirrielees, J., Crofford, L. J., Lin, Y., Kryscio, R. J., Dawson III, D. R., Ebersole, J. L., Miller, C. S.: Rheumatoid arthritis and salivary biomarkers of periodontal disease. J. Clin. Periodontol., roč. 37, 2010, č. 12, s. 1068-1074. Go to original source... Go to PubMed...
    17. Nieuw Amerongen, A., Bolscher, J. G. M., Veerman, E. C. I.: Salivary mucins: protective functions in relation to their heterogeneity. Glycobiology, 1995, č. 5, s. 733-740. Go to original source... Go to PubMed...
    18. Pruenster, M., Vogl, T., Roth, J., Sperandio, M.: S100A8/A9: from basic science to clinical application. Pharmacol. Ther., roč. 167, 2016, s. 120-131. Go to original source... Go to PubMed...
    19. Rai, B., Kaur, J., Anand, S. C., Jacobs, R.: Salivary stress markers, stress, and periodontitis: a pilot study. J. Periodontol., roč. 82, 2011, č. 2, s. 287-292. Go to original source... Go to PubMed...
    20. Rangé, H., Léger, T., Huchon, C., Ciangura, C., Diallo, D., Poitou, C., Chaussain, C.: Salivary proteome modifications associated with periodontitis in obese patients. J. Clin. Periodontol., roč. 39, 2012, č. 9, s. 799-806. Go to original source... Go to PubMed...
    21. Salazar, M. G., Jehmlich, N., Murr, A., Dhople, V. M., Holtfreter, B., Hammer, E., Kocher, T.: Identification of periodontitis associated changes in the proteome of whole human saliva by mass spectrometric analysis. J. Clin. Periodontol., roč. 40, 2013, č. 9, s. 825-832. Go to original source... Go to PubMed...
    22. Sandholm, L., Tolo, K., Olsen, I.: Salivary IgG, a parameter of periodontal disease activity? J. Clin. Periodontol., roč. 14, 1987, č. 5, s. 289-294. Go to original source... Go to PubMed...
    23. Séguier, S., Gogly, B., Bodineau, A., Godeau, G., Brousse, N.: Is collagen breakdown during periodontitis linked to inflammatory cells and expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human gingival tissue? J. Periodontol., roč. 72, 2001, č. 10, s. 1398-1406. Go to original source... Go to PubMed...
    24. Siqueira, W. L., Oppenheim, F. G.: Small molecular weight proteins/peptides present in the in vivo formed human acquired enamel pellicle. Arch. Oral Biol., roč. 54, 2009, č. 5, s. 437-444. Go to original source... Go to PubMed...
    25. Slots, J.: Periodontology: past, present, perspectives. Periodontology, 2000, roč. 62, 2013, č. 1, s. 7-19. Go to original source... Go to PubMed...
    26. Taylor, J. J.: Protein biomarkers of periodontitis in saliva. ISRN Inflammation, 2014. doi: 10.1155/2014/593151. Go to original source... Go to PubMed...
    27. Trindade, F., Oppenheim, F. G., Helmerhorst, E. J., Amado, F., Gomes, P. S., Vitorino, R.: Uncovering the molecular networks in periodontitis. Proteomics Clin. Appl., roč. 8, 2014, č. 9-10, s. 748-761. Go to original source... Go to PubMed...
    28. Wu, Y., Shu, R., Luo, L. J., Ge, L. H., Xie, Y. F.: Initial comparison of proteomic profiles of whole unstimulated saliva obtained from generalized aggressive periodontitis patients and healthy control subjects. J. Periodontal Res., roč. 44, 2009, č. 5, s. 636-644. Go to original source... Go to PubMed...
    29. Yoshizawa, J. M., Schafer, C. A., Schafer, J. J., Farrell, J. J., Paster, B. J., Wong, D. T.: Salivary biomarkers: toward future clinical and diagnostic utilities. Clin. Microbiol. Rev., roč. 26, 2013, č. 4, s. 781-791. Go to original source... Go to PubMed...
    30. Zhao, L., Lu, W.: Defensins in innate immunity. Curr. Opin. Hematol., roč. 21, 2014, č. 1, s. 37-42. Go to original source... Go to PubMed...
    31. Zhang, L., Henson, B. S., Camargo, P. M., Wong, D. T. The clinical value of salivary biomarkers for periodontal disease. Periodontol, 2000, roč. 51, 2009, č. 1, s. 25-37. Go to original source... Go to PubMed...
    32. van't Hof, W., Veerman, E. C., Nieuw Amerongen, A. V., Ligtenberg, A. J.: Antimicrobial defense systems in saliva. Monogr. Oral Sci., 2014, č. 24, s. 40-51. Go to PubMed...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content